产品简介:
Description
The RNAqueous?-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells (~100 cells to ~1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free? DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. Features of this kit:
* DNA-free RNA for RT-PCR
* Rapid, phenol-free total RNA isolation
* Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous?-4PCR Kit
The RNAqueous?-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater? Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Final Product Type: |
Total RNA |
| For Use With (Application): |
Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting |
| Isolation Technology: |
Silica Spin Column |
| Purification Target: |
Total RNA |
| Sample Type: |
Tissue, Cells |
| High-throughput Compatibility: |
Not High-throughput Compatible (Manual) |
| Product Line: |
Ambion?, RNAqueous? |
Contents & storage
* 600 μl 10X DNase I Buffer (-20°C)
* 45 μl DNase I 2 U/μl (-20°C)
* 1 ml Formaldehyde Load Dye (-20°C)
* 100 μl Linear Acrylamide 5 mg/ml (-20°C)
* 50 μl Positive Control Primers (-20°C)
* 1 ml 5M Ammonium Acetate (-20°C)
* 35 ml Water for 64% Ethanol (4°C)
* 600 μl DNase Inactivation Reagent (4°C)
* 30 ml Lysis/Binding Solution (4°C)
* 25 ml Wash Solution 1 (4°C)
* 35 ml Wash Solution 2/3 (4°C)
* 3 Pestles for tissue disruption (room temperature)
* 30 Filter Cartridges (room temperature)
* 60 Collection Tubes (room temperature)
* 25 ml Elution Solution (-20°C, 4°C, or room temperature)
For Research Use Only. Not for use in diagnostic procedures.
