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品名： | PureLink 96孔板血液、培养细胞RNA纯化试剂盒 |
产品简介:
Description
The PureLink? Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.
Improved plate design
The PureLink? Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Final Product Type: |
Total RNA |
| For Use With (Application): |
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting |
| For Use With (Equipment): |
Biomek? FX, Robotic Liquid Handling Systems, Vacuum Manifold, Eppendorf epMotion, Centrifuge, TECAN Freedom Evo, TECAN Genesis |
| Isolation Technology: |
Silica Plate |
| No. of Reactions: |
96 Preps |
| Purification Target: |
Total RNA |
| Purification Time: |
20 min |
| Sample Type: |
Bacteria, Blood Samples, Cell Cultures, Insect Cells, Mammalian Cells, Plant, Tissue (Mammalian), Yeast, Blood |
| Scale: |
Mini |
| Format: |
96-well plate |
| Quantity: |
1 kit |
| Yield: |
33 μg |
| Elution Volume Max: |
150 μl |
| Elution Volume Min: |
50 μl (centrifuge), 100 μl (vacuum) |
| High-throughput Compatibility: |
High-throughput Compatible |
| Purity or Quality Grade: |
Molecular Biology Grade |
| Shipping Condition: |
Room Temperature |
| System Type: |
PureLink? |
| Product Line: |
PureLink? |
Contents & storage
The PureLink? Pro 96 total RNA Purification Kit is supplied includes PureLink 96 Well Total RNA Filter Plates, elution plates, lysis buffer, Wash Buffers I and II, and RNase-free water. Components are also available separately. Store at room temperature. Guaranteed stable for 2 years when properly stored.
For Research Use Only. Not for use in diagnostic procedures.
