产品简介:
Description
The RNAqueous?-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free? DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:
* Highly concentrated RNA—for increased sensitivity in downstream assays
* Isolation of high-quality RNA from micro-scale samples
* Reagents/protocol for RNA recovery from LCM samples
How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free? system included in the kit. The RNAqueous?-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Final Product Type: |
Total RNA |
| For Use With (Application): |
Laser Capture Microdissection (LCM), Reverse Transcriptase PCR (RT-PCR), Northern Blotting |
| Isolation Technology: |
Silica Spin Column |
| Purification Target: |
Total RNA |
| Sample Type: |
Cell Cultures, Laser Capture Microdissection (LCM), Cultured Cells |
| Scale: |
Micro |
| Elution Volume Max: |
20 μl |
| High-throughput Compatibility: |
Not High-throughput Compatible (Manual) |
| Product Line: |
Ambion?, RNAqueous? |
Contents & storage
* 50 Micro Filter Cartridge Assembly (room temperature)
* 50 Micro Elution Tubes (room temperature)
* 15 ml Wash Solution 1 Concentrate (room temperature)
* 28 ml Wash Solution 2/3 concentrate (4°C)
* 25 Lysis Solution (4 °C)
* 200 μl LCM Additive (-20°C)
* 55 μl DNase 2 U/μl (-20°C)
* 600 μl 10X DNase I Buffer (-20°C)
* 150 μl DNase Inactivation Reagent (-20°C)
* 5 ml Elution Solution (-20°C, 4°C, room temperature)
For Research Use Only. Not for use in diagnostic procedures.
