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Description

Plant RNA Reagent is a single-component solution optimized for the isolation of high yields of high-quality total RNA from a variety of plant tissues, especially those rich in polyphenolics (e.g., conifer needles) or starch (e.g., potato tuber or seeds). Features of the Plant RNA Reagent:

* Isolate high-quality RNA from difficult plant materials
* Use for small-scale and large-scale RNA isolations

Improved quality and purity of plant RNA
Plant RNA Reagent is optimized to have less sample DNA contamination, to be twice as effective as TRIzol? Reagent, and have a simplified RNA extraction protocol for minimized time and labor. The end result is high-yield, high-purity RNA (determined by A₂₆₀/A₂₈₀ and gel analysis) from a variety of plant material sources.

Works for milligram to gram quantities of starting material
Plant RNA Reagent provides greater flexibility for sample size and processing formats, and can be used for both small-scale (up to 0.1 g plant tissue) and large-scale (0.1–5 g plant tissue) total RNA isolation. The amount supplied (100 mL) is sufficient for 200 reactions using 100 mg of tissue, or 4 reactions using 5 g of tissue.

Isolated RNA is ideal for a variety of applications and products:

* cDNA library construction kits
* Real-Time PCR (qPCR)
* Northern blotting kits & reagents
* Poly(A) selection kit
* RNase control reagents & assays

Specifications

Final Product Type:	Total RNA
For Use With (Application):	Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting
Isolation Technology:	Organic Extraction
No. of Reactions:	200 Preps
Purification Target:	Total RNA
Purification Time:	1 hr
Sample Type:	Plant Samples
Scale:	Scaleable
Quantity:	1
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Starting Material Amount:	Up to 1 g tissue, Up to 1 x 10^7 cells

Contents & storage