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品名： | RNAqueous-96孔板 总 RNA 提取试剂盒 |
产品简介:
Description
The RNAqueous?-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains sufficient reagents for 192 (2 x 96) RNA purifications. The RNAqueous?-96 Kit is ideal for the isolation of total RNA from multiple samples and very small samples.
Using the RNAqueous?-96 Kit
The procedure uses an RNA-binding glass-fiber filter and either vacuum filtration or centrifugation methods to provide high yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. The 96-well plates are compatible with most common 96-well plate vacuum manifolds and 96-well plate centrifuge rotors. The RNAqueous?-96 Kit provides consistently high yields of RNA from various amounts of starting material. The high quality of RNA isolated is illustrated (see figure).
Accessory product
The RNAqueous?-96 Automated Kit (Cat. No. AM1812) is the same product in a format suitable for automation.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Final Product Type: |
Total RNA |
| For Use With (Application): |
Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting |
| For Use With (Equipment): |
96-Well Plate Centrifuge Rotors, Vacuum Manifold (96-Well Plate) |
| Isolation Technology: |
Vacuum Filter Plate (Glass Fiber Filter) |
| Purification Target: |
Total RNA |
| Sample Type: |
Mammalian Cells, Tissue (Mammalian) |
| Format: |
96-well plate |
| High-throughput Compatibility: |
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols |
| Product Line: |
Ambion?, RNAqueous? |
Contents & storage
Store Nuclease–free Water, Elution Solution, Filter Plates, Collection Plates, Adhesive Plate Sealers, and Plate Lid at room temperature. Store Lysis?Binding Solution, Wash Solution Concentrate, and Rebinding Mix at 4°C. Store DNase I Buffer and DNase I at -20°C.
For Research Use Only. Not for use in diagnostic procedures.
