产品简介:
Description
The Ambion? RNAqueous?-Midi Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 107-108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous?-Midi Kit provides enough reagents for 15 RNA purifications.
* Convenient syringe-based filtration
* Compatible with vacuum manifolds when processing multiple samples The Ambion? RNAqueous?-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Final Product Type: |
Total RNA |
| For Use With (Application): |
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction |
| Isolation Technology: |
Syringe Filter (Glass Fiber) |
| Purification Target: |
Total RNA |
| Sample Type: |
Tissue, Cells |
| Includes: |
Formaldehyde Load Dye, Lysis/Binding Solution, Water for 64% Ethanol, Lithium, Chloride Solution, Wash Solution No. 1 and Wash Solution 2/3 Concentrate, Syringe Filter Units, Collection Tubes and Elution Solution |
| Product Type: |
Kit |
| High-throughput Compatibility: |
Not High-throughput Compatible (Manual) |
| Shipping Condition: |
Room Temperature |
| Product Line: |
Ambion?, RNAqueous? |
Contents & storage
Formaldehyde Load Dye should bestored at -20°C; Lysis/Binding Solution, Water for 64% Ethanol, LithiumChloride Solution, Wash Solution #1 and Wash Solution 2/3 Concentrate should bestored at 4°C; Syringe Filter Units, Collection Tubes and Elution Solution canbe stored at room temperature. All reagents are guaranteed stable for 6 months whenproperly stored.
For Research Use Only. Not for use in diagnostic procedures.
