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描述

Thermo Scientific Lambda Exonuclease is a highly processive 5'→3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA.

Highlights

* Active in Thermo Scientific PCR buffers

Applications
* Generating single-stranded PCR products for use in: * DNA sequencing
* Analysis of DNA single-strand conformation polymorphism (SSCP)
* Rolling circle amplification

* Producing single-stranded DNA from double-stranded DNA fragments
* Cloning of PCR products

Note

Use of this enzyme in certain applications may be covered by patents and may require a license.