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规格

Enzyme:	Nuclease

描述

Thermo Scientific Micrococcal Nuclease (S7 Nuclease) is an endo-exonuclease that digests single-stranded and double-stranded DNA and RNA.
Micrococcal Nuclease (S7 Nuclease) is a relatively nonspecific endo-exonuclease that digests single-stranded and double-stranded nucleic acids, but is more active on single-stranded substrates. Cleavage of DNA or RNA occurs preferentially at AT or AU-rich regions, yielding mononucleotides and oligonucleotides with terminal 3'-phosphates. The enzyme's activity is strictly dependent on Ca2+.
Applications

* Hydrolysis of nucleic acids in crude cell-free extracts
* Sequencing of RNA (see? Reference 2)
* Studies of chromatin structure
* A model for protein folding and for structure-function studies

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