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规格

数量:	100U
Enzyme:	RNase

描述

Thermo Scientific Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.

Applications

* Removal of mRNA prior to synthesis of second strand cDNA
* RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
* Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
* Site-specific cleavage of RNA
* Studies of in vitro polyadenylation reaction products

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