产品简介:
描述
Thermo Scientific Ribonuclease I (RNase I) is an endoribonuclease that preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2', 3'-cyclic monophosphate intermediates.
The enzyme does not require any metal ions for activity.
Highlights
* Stable and active under a wide variety of reaction conditions
* Can be heat inactivated in 30 minutes at 100°C
Applications
* Removal of RNA from DNA solutions
* Removal of RNA from recombinant protein preparations
* Ribonuclease protection assays
Notes
Mammalian ribonuclease inhibitors have no effect on RNase I. RNase I binds to DNA, but does not degrade it. RNase I amino acid sequence is typical for the RNase T2 family. Non-ionic detergents (e.g., Triton X-100) do not inhibit RNase I, and may even slightly stimulate its activity and stabilize it against heat inactivation. Triton X-100 or BSA (at 0.1 mg/mL) may prevent RNase I from sticking to glass vessels when working with dilute solutions. Polyamines stimulate the activity of RNase I.
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
