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描述

Thermo Scientific RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues. It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.

RNase T1 does not require metal ions for activity.

Applications

* Removal of RNA from DNA preparations
* RNA sequencing
* Ribonuclease protection assays. Used in conjunction with RNase A
* Removal of RNA from recombinant protein preparations
* Determination of the level of RNA transcripts synthesized in vitro from DNA templates containing a 'G-less cassette'