产品简介:
Description
The S.N.A.P.? MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10-100 ml of overnight culture (Figure 1). The S.N.A.P.? MidiPrep procedure takes just 40 minutes. You simply:
* Lyse cells and filter debris with the S.N.A.P.? filtering column
* Apply flow-through to the S.N.A.P.? DNA binding column
* Wash the column
* Elute pure, supercoiled plasmid DNA in water or TE buffer
Plasmid DNA purified with the S.N.A.P.? MidiPrep Kit is ready to use right off the column. No precipitation is necessary. Plasmid is eluted in TE or water at ~200 ng/μl-perfect for subsequent applications including mammalian cell transfection. Typical yields are shown in Table 1.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Final Product Type: |
Plasmid DNA |
| For Use With (Application): |
Next-Generation Sequencing, Cloning, Sequencing, Transformation, Transfection, In Vitro Transcription |
| Isolation Technology: |
Spin Column |
| Purification Target: |
Plasmid DNA |
| Sample Type: |
Bacterial Culture |
| High-throughput Compatibility: |
Not High-throughput Compatible (Manual) |
| Prep Scale: |
100-200 μg (Medium-Scale) Plasmid DNA |
| Product Line: |
S.N.A.P. ? |
Contents & storage
Store the S.N.A.P.? MidiPrep filtering columns, S.N.A.P.? MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, and 4X final wash at room temperature. Store the resuspension buffer, precipitation salt, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.
