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品名： | Exo-Resistant Random Primer |
产品简介:
描述
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'→5' exonuclease activity of proofreading DNA polymerases, such as Klenow Fragment and phi29 DNA Polymerase. It also has 5'- and 3'-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.
Highlights
* 3’-terminal PTO modifications protect from 3’→5’ exonuclease degradation and ensure efficient priming of DNA synthesis
Applications
* Strand displacement amplification of genomic DNA (see? Reference 2), plasmids and phage DNA
* DNA labeling by random priming (see? References 4-6)
Note
* Use of this enzyme in certain applications may be covered by patents and may require a license.
For Research Use Only. Not for use in diagnostic procedures.
