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品名： | T3 promoter Sequencing Primer, 24-mer |
产品简介:
描述
Thermo Scientific Transcription Promoter Sequencing Primers are single-stranded oligonucleotides with 5'- and 3'-hydroxyl ends. The primers are complementary to the T3 RNA Polymearse promoter region and are supplied as 10 μM aqueous solutions.
Applications* Sequencing of DNA fragments located downstream from the T3 RNA polymerase promoter sequence in common cloning vectors, such as
pTZ19R, pTZ57R, and pBluescript II
Quality Control* Functionally tested by dideoxy sequencing of a vector containing an appropriate RNA polymerase promoter sequence.
Catalog and Promoter sequences:* SO119—T3 promoter sequencing primer, 17-mer 5'-d (ATTAACCCTCACTAAAG)-3'
* SO120—T3 promoter sequencing primer, 24-mer 5'-d (GCGCGAAATTAACCCTCACTAAAG)-3'
Note* Primers cannot be used for certain plasmids that contain truncated, but still fully functional promoters. Primers are not phosphorylated.
Related ProductsSP6 promoter Sequencing Primer, 18-merT3 promoter Sequencing Primer, 17-merSP6 promoter Sequencing Primer, 24-merT7 promoter Sequencing Primer, 20-mer
For Research Use Only. Not for use in diagnostic procedures.
