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品名: | SP6 promoter Sequencing Primer, 24-mer |
产品简介:
描述
Thermo Scientific Transcription Promoter Sequencing Primers are single-stranded oligonucleotides with 5'- and 3'-hydroxyl ends. The primers are complementary to the SP6 RNA Polymerase promoter region and are supplied as 10 μM aqueous solutions.
Applications* Sequencing of DNA fragments located downstream from the SP6 RNA polymerase promoter sequence in common cloning vectors, such as
pTZ19R, pTZ57R, and pBluescript II
Quality Control* Functionally tested by dideoxy sequencing of a vector containing an appropriate RNA polymerase promoter sequence.
Catalog and Promoter sequences:* SO116—SP6 promoter sequencing primer, 18-mer 5'-d (ATTTAGGTGACACTATAG)-3'
* SO117—SP6 promoter sequencing primer, 24-mer 5'-d (CATACGATTTAGGTGACACTATAG)-3'
Note* Primers cannot be used for certain plasmids that contain truncated, but still fully functional promoters. Primers are not phosphorylated.
Related ProductsSP6 promoter Sequencing Primer, 18-merT7 promoter Sequencing Primer, 20-merT3 promoter Sequencing Primer, 17-merT3 promoter Sequencing Primer, 24-mer
For Research Use Only. Not for use in diagnostic procedures.