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品名： | SuperSignal West Femto Maximum Sensitivity Substrate |
产品简介:
规格
| Product Line: |
SuperSignal? |
| Recommended Antibody Concentrations: |
2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL) |
| Signal Duration: |
6 to 8 hr |
| Substrate Properties: |
Chemical Substrate |
| Detection Method: |
Chemiluminescent |
| 底物类型: |
HRP (Horseradish Peroxidase) Substrate |
| 试剂盒内容: |
Stable Peroxide Buffer, 10mL |
| 灵敏度: |
Low to mid-femtogram |
内容及储存
Sufficient For: 2 mini-gel blots; 200 cm2 of membrane
* Luminol/Enhancer, 10 mL
* Stable Peroxide Buffer, 10 mL
Store reagents at 4°C.
描述
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme.
SuperSignal West Femto Maximum Sensitivity Substrate characteristics include:
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Sensitivity: low-femtogram (mid-zeptomole)
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Stability: 8-hr working solution stability, 1-year kit stability at 4°C
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Compatibility: nitrocellulose and PVDF membranes
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Signal duration: up to 8 hours
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Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
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Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)
Compare and learn more about our western blot chemiluminescent substrates ?View all HRP substrates available ?SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
