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品名： | Pierce CN/DAB Substrate Kit |
产品简介:
规格
| Product Line: |
Pierce? |
| Substrate Properties: |
Chemical Substrate |
| Detection Method: |
Colorimetric |
| 底物类型: |
HRP (Horseradish Peroxidase) Substrate |
内容及储存
Sufficient For: 25 uses at 10 mL each
* CN/DAB (10X), 25 mL
* Stable Peroxide Buffer, 250 mL
Store at 4°C.
描述
Thermo Scientific Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.
This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.
Features of the CN/DAB Substrate Kit:
* HRP substrate—for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)
* Chromogenic—no special equipment needed for visualization; yields intense black bands that are easy to photograph
* Easy-to-use kit—substrate stock solution and stable peroxide substrate buffer mean that there's no dry powders to measure and dissolve
* Stable—store refrigerated at 4°C for at least one year
Includes:
* CN/DAB Solution (10X) and Stable Peroxide Substrate Solution (1X)
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
