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品名： | EGS (ethylene glycol bis(succinimidyl succinate)) |
产品简介:
规格
| Product Line: |
Pierce? |
| Crosslinker Type: |
Homobifunctional |
| Labeling Method: |
Chemical Labeling |
| Reactive Moiety: |
NHS Ester |
| Solubility: |
DMSO (Dimethylsulfoxide), DMF (Dimethylformamide) |
| Spacer: |
Cleavable, Long |
内容及储存
Store at -20°C. Shipped at ambient temperature.
描述
Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.
Features of the EGS crosslinker:
*
Reactive groups: NHS ester (both ends)
*
Reactive towards: amino groups (primary amines)
* Water-insoluble (dissolve first in DMF or DMSO); compare to
Sulfo-EGS* Membrane-permeable, allowing for intracellular crosslinking
* Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
* Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS
Product References:Crosslinker Application Guide -- search for recent literature references for this product
Related ProductsEGS (ethylene glycol bis(succinimidyl succinate))
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
