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品名: | North2South Biotin Random Prime DNA Labeling Kit |
产品简介:
规格
Product Line: |
North2South? |
内容及储存
Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)
* Heptanucleotide Mix (5X), 100 μL
* Deoxynucleotide Mix (5X), 80 μL
* Reaction Buffer (10X), 50 μL
* DNA Polymerase Klenow Fragment (5U/μL), 10 μL
* Control DNA (250 ng/μL), 5 μL
* Ammonium Acetate (5M), 1 mL
* EDTA (0.5M, pH 8.0), 1 mL
* Nuclease-free Water, 1 mL
* Biotin-11-dUTP, 20 μL
Store all components at -20°C.
描述
Thermo Scientific North2South Random Prime DNA Biotinylation Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridization and detection methods.
Features of the North2South Random Prime DNA Biotinylation Kit:
* Works with as little as 100ng starting DNA template
* Exonuclease-free Klenow fragment in the DNA labeling kit produces higher yields
* Each reaction yields probe sufficient for approximately three (10 x 10 cm) blots
This random prime DNA labeling kit is based on the procedure of Feinberg and Vogelstein (Ref.4,5). Random heptanucleotides containing all possible sequences are annealed to a denatured DNA template. These act as primers for complementary strand synthesis by DNA Polymerase (Klenow fragment, 3'-5' exo-). Biotinylated dNTPs in the reaction mix are incorporated into the newly synthesized DNA. This protocol yields biotin-labeled DNA of high activity for use as probes in hybridization experiments such as Southern and Northern hybridizations. The resulting hybrids can be detected with streptavidin-horseradish peroxidase (HRP) and a chemiluminescent substrate kit such as
North2South Chemiluminescent Hybridization and Detection Kit.
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.