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品名:North2South Biotin Random Prime DNA Labeling Kit
产品简介:

规格

Product Line: North2South?

内容及储存

Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)
* Heptanucleotide Mix (5X), 100 μL
* Deoxynucleotide Mix (5X), 80 μL
* Reaction Buffer (10X), 50 μL
* DNA Polymerase Klenow Fragment (5U/μL), 10 μL
* Control DNA (250 ng/μL), 5 μL
* Ammonium Acetate (5M), 1 mL
* EDTA (0.5M, pH 8.0), 1 mL
* Nuclease-free Water, 1 mL
* Biotin-11-dUTP, 20 μL

Store all components at -20°C.

描述

Thermo Scientific North2South Random Prime DNA Biotinylation Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridization and detection methods.

Features of the North2South Random Prime DNA Biotinylation Kit:

* Works with as little as 100ng starting DNA template
* Exonuclease-free Klenow fragment in the DNA labeling kit produces higher yields
* Each reaction yields probe sufficient for approximately three (10 x 10 cm) blots

This random prime DNA labeling kit is based on the procedure of Feinberg and Vogelstein (Ref.4,5). Random heptanucleotides containing all possible sequences are annealed to a denatured DNA template. These act as primers for complementary strand synthesis by DNA Polymerase (Klenow fragment, 3'-5' exo-). Biotinylated dNTPs in the reaction mix are incorporated into the newly synthesized DNA. This protocol yields biotin-labeled DNA of high activity for use as probes in hybridization experiments such as Southern and Northern hybridizations. The resulting hybrids can be detected with streptavidin-horseradish peroxidase (HRP) and a chemiluminescent substrate kit such as North2South Chemiluminescent Hybridization and Detection Kit.
For Research Use Only. Not for use in diagnostic procedures.
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