| |
|---|
|
品名： | Pierce Gaussia Luciferase Glow Assay Kit |
产品简介:
规格
| Assay: |
Reporter Enzyme, Luciferase Reporter Assay |
| Compatible Cells: |
Mammalian Cells |
| Detection Method: |
Bioluminescent |
| 适用于（设备）: |
Luminometer (Microplate) |
| 形式: |
384-well plate, 96-well plate |
| Label Type: |
Enzyme Labeled |
| Product Line: |
Pierce? |
| 底物: |
Coelenterazine |
| Substrate Properties: |
Chemical Substrate |
| 基质类型: |
Luciferase Substrate |
| Target Enzyme: |
Luciferase, Gaussia Luciferase |
| 技术.: |
Enhanced Chemiluminescence |
| 数量: |
100 rxn kit |
内容及储存
Sufficient For: 100 luciferase assays in microplate wells
* Gaussia Glow Assay Buffer, 5 mL (store at 4°C)
* 100X Coelenterazine, 50 μL (store at -80°C)
* Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)
Kit may be stored at -80°C, or individually at indicated temperatures.
描述
The Thermo Scientific Pierce Gaussia Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour), especially in the presence of Gaussia-Dura luciferase reporter.
Features of the Gaussia Luciferase Glow Assay Kit:
* Sensitive—highly sensitive detection of Gaussia luciferase activity
* Stable—increased signal stability
* Simple—does not require a luminometer with injectors
* Compatible—assay reagents compatible with other Gaussia luciferases
* Automation-friendly—amenable to high throughput assays
* Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
* Safe—allows one to perform non-radioactive assays
This Pierce Luciferase Glow Assay Kit contains reagents for measuring Gaussia luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia-Dura Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity with extended signal stability. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Gaussia luciferases or derivatives that use coelenterazine as a substrate.
Includes:
Cell lysis buffer, reaction buffer and substrate
Requires:
Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
Applications:
* Promoter studies for analyzing cis- regulatory elements and trans-acting factors
* Drug screening
* siRNA and miRNA screening
* Multiplexed assays to study off-target effects
* Secretory pathway/protein localization reporter assays
* Signal transduction pathway analysis
* RNA splicing studies
Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The signal produced by Gaussia luciferase shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions.
The Gaussia-Dura gene used in the Thermo Scientific Gaussia-Dura Luc Vectors is derived from the wild type Gaussia gene and provides equally bright but more stable light output. The Gaussia Glow Assay Kit reagent yields a Gaussia-Dura luciferase reaction with glow-type kinetics and is recommended for luminometers without injectors or for applications requiring batch processing of samples.
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
