产品简介:
Description
The PureLink? HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 μg plasmid DNA from 100–200 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. Advantages of the PureLink? HiPure Plasmid Maxiprep Kit include:
* Labor and time saving—no need for extra steps to remove endotoxin and other contaminants
* High-purity, low-endotoxin product—pure enough for mammalian cell transfection
* Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs
Anion-exchange chromatography for plasmid purification
The PureLink? HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal. Plasmid preparations can typically be completed in less than 2 hours.
Contaminant-free DNA
Unlike cesium chloride gradient protocols, PureLink? HiPure Plasmid Purification Systems do not use organic solvents, ethidium bromide, or cesium chloride, all of which are troublesome to work with and to dispose of. Typically, plasmid DNA prepared using the PureLink? HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications. Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Final Product Type: |
BAC DNA, Plasmid DNA |
| For Use With (Application): |
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription |
| Isolation Technology: |
Anion Exchange Resin with Lysate Clearing Filter |
| No. of Reactions: |
10 Preps |
| Purification Target: |
BAC DNA, Plasmid DNA |
| Purification Time: |
2 hrs |
| Sample Type: |
Bacterial Culture |
| Includes: |
100mL Resuspension buffer (R3), 550μL RNase A, 100mL lysis buffer (L7), 100mL precipitation buffer (N3), 300mL equilibration buffer (EQ1), 2 x 300mL wash buffer (W8), 200mL elution buffer (E4), 30mL TE buffer, 10 HiPure columns, 3 Column holders |
| Quantity: |
10 columns |
| Yield: |
1000 μg |
| Elution Volume Max: |
15 ml |
| Elution Volume Min: |
15 ml |
| High-throughput Compatibility: |
Not High-throughput Compatible (Manual) |
| Plasmid: |
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC |
| Prep Scale: |
> 200 μg (Large-Scale) Plasmid DNA |
| Purity or Quality Grade: |
Transfection Grade |
| Shipping Condition: |
Room Temperature |
| System Type: |
PureLink? |
| Product Line: |
PureLink? |
Contents & storage
* 100 ml Resuspension Buffer (R3)
* 550 μl RNase A
* 100 ml Lysis Buffer (L7)
* 100 ml Precipitation Buffer (N3)
* 300 ml Equilibration Buffer (EQ1)
* 2 × 300 ml Wash Buffer (W8)
* 200 ml Elution Buffer (E4)
* 30 ml TE Buffer
* 10 HiPure Columns
* 3 Column Holders
Store all components at room temperature.
For Research Use Only. Not for use in diagnostic procedures.
