产品简介:
Description
The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.
NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our
FastVac Vacuum Manifold.
Advantages of the PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit include:
*
Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
*
Low endotoxin plasmid—the resulting plasmid contains <1 EU/μg endotoxins
*
High yields—isolate up to 0.4 mg of high-quality plasmid DNA
*
Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Quantity: |
25 Preps |
| Prep Scale: |
Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg |
| Shipping Condition: |
Room Temperature |
| Product Line: |
PureLink? |
Contents & storage
* Resuspension buffer, store at 4°C upon reciept
* Lysis buffer, store at room temperature
* Precipitation buffer, store at room temperature
* Binding buffer, store at room temperature
* Wash buffer 1, store at room temperature
* Wash buffer 2 (concentrate), store at room temperature
* Elution buffer, store at room temperature
* Column assemblies, store at room temperature
* Syringe filters, store at room temperature
* Syringe plungers, store at room temperature
* Collection tubes, store at room temperature
For Research Use Only. Not for use in diagnostic procedures.
