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品名： | PureLink高品质96孔板质粒提取试剂盒 |
产品简介:
Description
The PureLink HQ 96 Plasmid DNA Purification Kit isdesigned for high-throughput isolation of high-qualityplasmid DNA from bacterial cells. It can beused with a vacuum manifold or a centrifuge and iscompatible with automated liquid handling workstations.
The PureLink HQ 96 Plasmid DNA Purification Kit offersthe following advantages:
* Ability to isolate up to 10 μg plasmid DNA per well
* Designed to isolate high-quality plasmid DNA in30-45 minutes
* Minimal genomic DNA contamination in the purifiedplasmid DNA
* Compatible with automated liquid handlingworkstations
* Reliable performance of the purified plasmid DNA indownstream applications such as mammaliantransfection
Process overview
Bacterial cells are harvested and resuspended in ResuspensionBuffer with RNase. The cells are lysed using an alkaline/SDScell lysis procedure. The lysate is then neutralized andconditions are adjusted for subsequent binding. After lysateclarification using the PureLink HQ 96 Clarification Plate, thelysate is processed through the PureLink HQ 96 BindingPlate. The DNA binds to the silica-based membrane in theplate and impurities are removed by washing with WashBuffer. The DNA is then eluted in low salt Elution Buffer orwater.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Final Product Type: |
Plasmid DNA |
| For Use With (Application): |
PCR, Transfection, Sequencing, Transformation, Cloning |
| Isolation Technology: |
Silica Plate |
| Purification Target: |
Plasmid DNA |
| Sample Type: |
Bacterial Culture |
| High-throughput Compatibility: |
High-throughput Compatible |
| Prep Scale: |
< 100 μg (Small-Scale) Plasmid DNA |
| Shipping Condition: |
Room Temperature |
| Product Line: |
PureLink? |
Contents & storage
Sufficient reagents are provided in the kit to perform 384(4 × 96) isolations.
Reagents include:
* Resuspension Buffer (R1)
* Lysis Buffer (L1)
* Neutralization/Binding Buffer (B1)
* Wash Buffer (W1)
* Elution Buffer; 10 mM Tris-HCl, pH 8.5 (E1)
* RNase A
* PureLink HQ 96 Clarification Plate
* PureLink HQ 96 Binding Plate
* Receiver Plate
* Adhesive Foil for plates
For Research Use Only. Not for use in diagnostic procedures.
