产品简介:
Description
The Easy-DNA? Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wide range of cells and tissue types. Sample sizes can range from a single hair follicle up to 1 gram of mammalian tissue. Other samples tested include mouse tails, plant leaves, yeast, and E. coli cells. These samples yield high-quality DNA with an average size between 100 kb and 200 kb, which is suitable for PCR, DNA hybridization, genomic DNA library construction, and other applications. Advantages of the Easy-DNA? Kit include:
* Reliability—high-quality genomic DNA from large or small samples of cells, tissue, plant, yeast, E. coli, blood, and even hair follicles
* Simplicity—for most sample types, only 4 steps, typically requiring less than 90 minutes are needed to isolate high-quality DNA
* Versatility—one kit is supported by optimized protocols for a variety of cell and tissue types
Simple workflow, quality results
Optimized protocols for a variety of cell and tissue types are included in the instruction manual supplied with the kit. Generally, small-scale gDNA preparations are performed in microfuge tubes, in which samples are mixed with Lysis Solution and incubated at 65°C for 10 minutes. After extraction with chloroform (not included in kit), precipitation, and reconstitution of the DNA pellet, the preparation is complete. The kit contains a protein degradation reagent (for use with high-protein–containing samples such as connective tissue), mussel glycogen for use as a co-precipitant for dilute DNA samples, and an RNase solution.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| For Use With (Application): |
Southern Blotting, PCR |
| Isolation Technology: |
Organic Extraction |
| Purification Target: |
Genomic DNA |
| Sample Type: |
Plant Samples, Bacteria, Cells, Tissue, Fungi, Yeast |
| High-throughput Compatibility: |
Not High-throughput Compatible (Manual) |
| Product Line: |
Easy-DNA? |
Contents & storage
The Easy-DNA? Kit contains enough reagents for 15–200 samples, depending on sample size and type.
This kit contains:
* 55 ml Solution A (Lysis Solution)
* 25 ml Solution B (Precipitation Solution)
* 100 ml TE Buffer
* 750 μl Mussel Glycogen (2 mg/ml in sterile water)
* 750 μl RNase (2 mg/ml in sterile water)
* 750 μl Protein Degrader (5 mg/ml in sterile water)
Store kit at room temperature. For long-term storage, Mussel Glycogen, RNase, and Protein Degrader may be stored at -20°C.
For Research Use Only. Not for use in diagnostic procedures.
