产品简介:
规格
| 适用于（应用）: |
PCR, Real-Time PCR |
| Product Line: |
RevertAid |
| Reaction Format: |
SuperMix or Master Mix |
| Reverse Transcriptase: |
ThermoScript |
| 样品类型: |
Cells |
| Shipping Condition: |
Dry Ice |
内容及储存
* 1 x 50 μL of 20X Enzyme mix (MuLV and RNase inhibitor protein)
* 1 x 500 μL of 2X RT Buffer Mix (includes dNTPs, random octamers, and Oligo dT-16)
Store at -15 to -25°C. 2X RT Buffer Mix can be stored at 2–8°C for up to 6 months.
描述
Thermo Scientific RevertAid Master Mix is a complete system for efficient synthesis of first-strand cDNA from mRNA or total RNA templates. The mix includes RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. RevertAid Master Mix provides all cDNA synthesis reaction components in a convenient two-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications. The two-tube formulation has a shorter reaction time and requires fewer pipetting steps than the RevertAid RT Reverse Transcription Kit, while maintaining high performance.
Highlights
* Fast—short reaction time (typically 30–60 min)
* Convenient—easy workflow with few pipetting steps
* Reliable—reverse transcription of both abundant and limited targets
* Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse-transcription reaction
Applications
First strand cDNA synthesis for RT-PCR and RT-qPCR
* Construction of full length cDNA libraries
* Antisense RNA synthesis
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
