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描述

Thermo Scientific MagJET NGS Cleanup and Size Selection kit, sufficient for 10 preps, is designed for efficient and robust DNA fragment library cleanup and size-selection from a variety of enzymatic reaction mixtures including PCR, ligation, adapter addition and DNA end-repair reaction mixes. The kit utilizes high-capacity MagJET paramagnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range. The purified DNA fragments are free of any next-generation sequencing workflow inhibiting components, such as sequencing adapters, primer dimers, unincorporated nucleotides, enzymes or salts, and are ready-to-use in downstream NGS applications. No prior DNA fragment library purification is required before the cleanup and size-selection procedures.

The kit is suitable for cleanup or size selection of 5 ng to 5 μg of DNA fragment library. MagJET cleanup and adapter removal protocols can easily be automated for higher throughput on automatic platforms such as Thermo Scientific KingFisher Flex and Thermo Scientific KingFisher Duo.

Highlights

* Robust—reliable cleanup and size selection from different reaction mixtures and buffers
* Flexible—user-defined size-selection of exact DNA fragment range
* NGS-grade purity—complete removal of NGS adapters, primers, nucleotides, enzymes and other reaction components inhibiting NGS workflow
* High throughput compatible—easy to automate on the Thermo Scientific KingFisher magnetic particle processors for high-throughput sample handling

Applications

DNA fragment library cleanup and size-selection for downstream use in next generation sequencing workflows.
Includes
* MagJET Magnetic Beads
* Binding Solution
* Wash Buffer (concentrated)
* Elution Buffer
* DNA Fragment Mix