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品名： | Pierce Glu-C Protease, MS Grade |
产品简介:
规格
| Detection Method: |
Mass Spectrometry |
| Final Product Type: |
Peptide |
| 适用于（设备）: |
Mass Spectrometer |
| Product Line: |
Pierce? |
| Starting Material: |
Protein samples, Cell Lysate |
| Workflow Step: |
Protein Digestion |
| 描述: |
Pierce Glu-C Endoproteinase, MS Grade |
内容及储存
Store at -20°C in a nonfrost-free freezer.
描述
Thermo Scientific Glu-C Protease, MS Grade, is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
Features of Glu-C Protease, MS Grade:
* Complementary to tryptic digests—hydrolyzes proteins specifically at the carboxyl side of glutamic acids
* Increased sequence coverage—better protein characterization results from overlapping peptides with complementary sequence coverage and chromatographic, ionization and fragmentation properties
* High specific activity—greater than 500 units/mg protein
* N-terminal arginine cleavage specificity—at least 90% for a complex protein sample
* Stable—provided in a lyophilized format
Product Details
This Glu-C is a mass spectrometry (MS)-grade serine protease isolated from Staphylococcus aureus. Glu-C can be used alone or alongside trypsin or other proteases to produce complementary protein digests for peptide mapping and protein sequencing. Glu-C protease is suitable for either in-solution or in-gel digestion workflows. This Glu-C enzyme is packaged lyophilized (5 x 10 μg).
The endoproteinase Glu-C, also referred to as V-8 Protease, specifically cleaves the carboxyl side of glutamic residues when reactions are carried out in ammonium bicarbonate and ammonium acetate buffers, generating a limited number of peptide fragments. Cleavage can also occur at both glutamic and aspartic residues in phosphate buffers. Glu-C can efficiently digest protein in 5-18 hours at 37°C. Glu-C Protease remains active under denaturing conditions such as 2M urea, 1M guanidine·HCl, 0.1% SDS, 2% CHAPS and 20% acetonitrile. Glu-C activity is optimal at pH 8. This lyophilized enzyme has a mass of 27 kDa and is stable for 1 year when stored at -20°C.
Applications
* Improved sequence coverage of protein digests
* In-solution digestion of proteins
* In-gel digestion of proteins
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
