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规格

样品类型:	Plasmid DNA
Cell Type:	Established Cell Lines, Primary Cells, Endothelial Cells, Epithelial Cells, Kidney, Macrophages, Myeloma Cells, Hepatocytes, Fibroblasts
分类分类:	Chemically Defined
Transfection Technique:	Chemical Transfection
Product Line:	TurboFect

描述

Thermo Scientific TurboFect Transfection Reagent is a highly efficient, easy-to-use, non-immunogenic transfection reagent for delivery of DNA plasmids and expression vectors into eukaryotic cells.

Features of TurboFect Transfection Reagent:

* High transfection efficiency of a wide variety of cell types
* No optimization required for most cell lines
* Excellent transfection efficiency in the presence or absence of serum
* Minimal cytotoxicity
* Ready-to-use—no need to reconstitute, dilute or manipulate

Applications of TurboFect Transfection Reagent :
* Stable and transient transfection or co-transfection of DNA or RNA
* Experiments with adherent or suspension cells, differentiated or undifferentiated cells
* Gene expression studies and assays involving GFP or luciferase reporters

TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. TurboFect Reagent interacts with DNA to form small, stable, highly diffusible complexes which are readily endocytosed. The reagent creates a proton-sponge effect that buffers endosomal pH by provoking massive proton accumulation and passive chloride influx. As a result, rapid osmotic swelling of the endocytosed DNA-complex causes it to rupture in the cytosol, allowing translocation of DNA to the nucleus. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells.

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