描述
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by
in vitro evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.
Highlights* Increased reaction temperatures—the first strand of cDNA can be synthesized within the 42 to 65°C temperature range
* High yields of full-length first strand cDNA—with RNA templates up to 20 kb
* Flexible priming—oligo(dT)
18, random hexamer, or gene-specific primers
Applications* First Strand cDNA synthesis for RT-PCR
* Construction of cDNA libraries
* Generation of probes for hybridization
* Antisense RNA synthesis
Includes* Maxima H Minus First Strand cDNA Synthesis Kit contains Maxima H Minus Enzyme Mix, Oligo(dT)
18 and Random hexamer primers, 5X RT Buffer, dNTP Mix, and nuclease-free water.
Additional information about reaction components* Maxima H Minus Enzyme Mix contains
Maxima H Minus Reverse Transcriptase and
RiboLock RNase Inhibitor. RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B and C at temperatures up to 55°C.
*
oligo(dT)18 and
random hexamer primers are supplied with the kit. Random hexamer primers bind non-specifically and are used to synthesize cDNA from all RNAs in a total RNA population. The oligo(dT)
18 primer selectively anneals to the 3'-end of poly(A) RNA, synthesizing cDNA only from poly(A) tailed mRNA. Gene-specific primers may also be used with the kit to prime synthesis from a specified sequence.
*
10 mM dNTP Mix is a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP.
*
Nuclease-free water is provided for reaction set-up and dilution of sample DNA. The absence of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.
For Research Use Only. Not for use in diagnostic procedures.
