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品名： | RevertAid H Minus Reverse Transcriptase |
产品简介:
规格
| Optimal Reaction Temperature: |
42-45° C |
| Product Line: |
RevertAid |
| Reverse Transcriptase: |
RevertAid H Minus |
| Ribonuclease H Activity: |
None |
| Size (Final Product): |
13 kb or less |
| 包括: |
1mL 5X Reaction Buffer |
内容及储存
RevertAid H Minus Reverse Transcriptase (200 U/μL) is supplied with 5X Reaction Buffer (250 mM Tris-HCl (pH 8.3 at 25°C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT).
Store at -20°C.
描述
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).
Highlights
* High yields of full-length first strand cDNA up to 13 kb
* Optimum activity at 42 to 45°C
* Active up to 55°C
* Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Applications
* First strand cDNA synthesis for RT-PCR and real-time RT-PCR
* Reverse transcription at elevated temperatures to reduce effects of secondary structure
* Synthesis of cDNA for cloning and expression
* Generation of labeled cDNA probes for microarrays
* DNA labeling
* Analysis of RNA by primer extension
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
